Journal: Bioactive Materials

Article Title: Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg 2+ signaling and neuropilin-1–mediated angiogenesis

doi: 10.1016/j.bioactmat.2026.02.031

Figure Lengend Snippet: Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) Transwell assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).

Article Snippet: For the Transwell assay, BMSCs were seeded in the upper chambers of Transwell inserts (8.0 μm pore size; Servicebio, China), while different culture conditions were applied in the lower chambers according to the experimental groups.

Techniques: Wound Healing Assay, Transwell Assay, Migration, Immunofluorescence, Staining, Labeling, Western Blot, Expressing

Journal: Frontiers in Immunology

Article Title: Multi-omics profiling identifies ADAM9 as a key efferocytosis driver in lung adenocarcinoma

doi: 10.3389/fimmu.2026.1772167

Figure Lengend Snippet: Effects of ADAM9 knockdown in macrophage cells. (A) Relative mRNA expression of ADAM9 in LUAD tissues and adjacent tissues. (B) ADAM9 expression in LUAD tissues evaluated by IHC. (C, D) ADAM9 expression in M2 macrophages transfected with siRNA. (E) RT-qPCR analysis of M2 polarization markers in si-ADAM9-transfected M2 macrophages. (F) ATP content assay in M2 macrophages transfected with si-ADAM9. (G) ADAM9 mRNA expression in normal bronchial epithelial cells and LUAD cell lines. (H) Transwell migration assay showing the migratory ability of A549 and H1975 cells co-cultured with M2 macrophages. (I, J) EdU assay detecting the proliferative capacity of LUAD cells co-cultured with M2 macrophages. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Cells were pre-cultured in serum-free medium for 48 h. Then, 2.0×10 4 cells were seeded in the upper chamber of a transwell insert (NEST Biotechnology, Wuxi, China), while the lower chamber was filled with RPMI-1640 medium containing 10% FBS (Bioland, China).

Techniques: Knockdown, Expressing, Transfection, Quantitative RT-PCR, Transwell Migration Assay, Cell Culture, EdU Assay